Properties of Polysaccharides Extracted from P hellinus Linteus Using High Hydrostatic Pressure Processing and Hot Water Treatment

Extraction of medicinal polysaccharides from Phellinus linteus using hot water treatment alone and a combination of high hydrostatic pressure processing (HHP) with hot water treatment was investigated with regard to the yield, medicinal value and structural changes of the extracts. The maximum yield of polysaccharides and the highest content of β‐glucan were obtained using HHP (300 MPa, 50C, 10 min) combined with hot water treatment (65C, 3 h). Hexose sugars were present in higher molar concentrations compared with pentose sugars regardless of the extraction condition used, although the amino acid composition of the polysaccharide samples varied depending on the extraction procedure. Structural analysis using FT‐IR (Fourier transform infrared) and 1H NMR (proton nuclear magnetic resonance) spectroscopy showed that the extracts were composed of protein‐bound polysaccharides linked by β‐glycosidic bonds, which are critical for the physiological properties of polysaccharides, and that the absorption intensity of the HHP plus hot water extract was higher than that of the extract obtained by hot water alone (95C, 6 h). The results from this study suggest that the combination processing at high hydrostatic pressure and hot water treatment is efficient for extracting polysaccharides from P. linteus as these two treatments provide a synergistic effect. Practical Application Phellinus linteus, an edible medicinal mushroom, has various biological activities such as immunostimulatory and antitumor activities. This study investigated the utility of HHP for extracting medicinal components from P. linteus with regard to yield, medicinal value and structural changes. The results of this study could expand the application of HHP in the development of medicinal mushroom products.