Magnitude of Genetic Diversity of Fenugreek (Trigonillafoenum-Gracum) Germplasm Based on Agronomic Traits and Biochemical Markers

The present study was aimed to evaluate the level of genetic diversity in germplasm of Fenugreek, to evaluate Fenugreek germplasm for Agro-morphological trait and SDS-PAGE and Estimation of genetic diversity in Trigonlla foenum-gracum germplasm to select elite genotypes for utilization in the breeding programs.In the preliminary study of genetic diversity in local germplasm evaluation of Trigonellafoenum greacum germplasm consisting of 55 accessions, was evaluated for qualitative and quantitative characters.For qualitative characters a significant in consistency was perceived for traits of plant, shape and types of plants.Correlation analysis for quantitative traits revealed that, significant correlations were observed for different characters for all accessions studied and the result could be implied in wider perspectives.In the current studies, Intraspecific variations was not high and it was evaluate that SDS-PAGE alone did not exihibit high level of intraspecific variations, as a result varied accessions depends on SDS-PAGE and recommended to be attained from alterations, preferably from centre of diversity to build a broad based gene pool with maximum variability.SDS-PAGE was conducted for 55 genotypes of the base of qualitative and quantitative traits.SDS-PAGE Marker proved to be valid biochemical technique for determining genetic diversity and variability.The grouping on the base of altitude, crop ecological zones and physio geo-graphical regions of Pakistan could not prove its worth for the present Trigonella foenumgreacum used.Further, cluster analysis showed that the breeders or transport of grains to different markets from where the seed of various is disseminated throughout the country grouped many accessions from same origin separately that may be because of frequent exchange of germplasm.