Key result
Inhibiting eIF4A blocks VPg-dependent porcine sapovirus translation and infectivity despite a short 5' UTR.
eIF4A is essential for porcine sapovirus translation, highlighting its role in unwinding viral RNA structures.
eIF4A inhibition may block sapovirus replication; hypothesis-generating for host-targeted antivirals in veterinary settings.
The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.
No takes yet. Share an insight, caveat, or question.
Hosmillo et al. (2016) studied Porcine sapovirus infection. eIF4A inhibitors and dominant-negative mutants was evaluated on Viral translation and infectivity. Inhibition or mutation of eIF4A demonstrated that the helicase is required for porcine sapovirus VPg-dependent translation and infectivity despite a very short 5' UTR.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: