What question did this study set out to answer?

To determine whether tumor-targeted superantigen fusion proteins can recruit tumor-infiltrating T lymphocytes and eradicate established micrometastases in vivo.

October 10, 1995Proceedings of the National Academy of SciencesOpen Access

Antibody-targeted superantigens are potent inducers of tumor-infiltrating T lymphocytes in vivo.

View Full Paper

Ask AI

Bookmark

Authors

MDMikael DohlstenLund University JHJohan HanssonUppsala University LOLennart OhlssonStockholm University

Discussion

Member takes

Implication

Preclinical animal study demonstrates that antibody-targeted superantigens eliminate lung micrometastases in mice, indicating a potent method to recruit tumor-infiltrating T cells.

Key Points

To determine whether tumor-targeted superantigen fusion proteins can recruit tumor-infiltrating T lymphocytes and eradicate established micrometastases in vivo.
Injected C57BL/6 mice intravenously with C215 antigen-transfected syngeneic B16 melanoma cells to establish lung micrometastases.
Treated mice bearing 5-day-old metastases with an anti-C215 Fab fragment-staphylococcal enterotoxin A (C215Fab-SEA) fusion protein, evaluating efficacy in immunocompetent versus T-cell-deficient nude mice.
Depleted specific T-cell subsets using monoclonal antibodies and quantified tumor infiltration and cytokine expression via immunohistochemical analysis.
Treatment with C215Fab-SEA resulted in more than 90% reduction in the number of established 5-day lung metastases in immunocompetent mice, but produced no therapeutic effect in T-cell-deficient nude mice.
C215Fab-SEA induced massive intratumoral infiltration of CD8+ and CD4+ T cells along with strong local expression of tumor necrosis factor alpha and interferon gamma, whereas SEA alone caused only general lung inflammation without tumor-specific infiltration.

Cite This Study

Dohlsten et al. (1995) studied this question.

synapsesocial.com/papers/6aa44132e39e00092d1aa9d4 https://doi.org/10.1073/pnas.92.21.9791

View Full Paper

Ask AI

Bookmark

Implication

Preclinical animal study demonstrates that antibody-targeted superantigens eliminate lung micrometastases in mice, indicating a potent method to recruit tumor-infiltrating T cells.

Antibody-targeted superantigens are potent inducers of tumor-infiltrating T lymphocytes in vivo.

Discussion

Member takes

Implication

Key Points

Cite This Study

Antibody-targeted superantigens are potent inducers of tumor-infiltrating T lymphocytes in vivo.

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider