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A rapid and simple high performance thin layer chromatographic method has been developed for the simultaneous quantitation of pharmacologically important diaryl heptanoids curcumin, demethoxy curcumin, and bis-demethoxy curcumin in Curcuma longa and C. amada. The assay combines the isolation and separation of curcuminoids on silica gel 60F254 high performance thin layer chromatographic plates, followed by scanning of the spots at 366 nm using a UV detection mode.
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Gupta et al. (1999) studied this question.
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