Key result
In vitro incubation of rat thoracic aorta with high glucose for 3 hours preserved and increased A23187-mediated contractile responses compared to normoglycemic conditions where responses were significantly depressed.
Why the study?
To investigate exposure-time-dependent changes in endothelium-derived contractile responses under normoglycemic and hyperglycemic conditions.
Does acute in vitro hyperglycemia alter A23187-mediated contractile responses in isolated rat thoracic aorta?
Does acute in vitro hyperglycemia alter A23187-mediated contractile responses in isolated rat thoracic aorta?
Acute hyperglycemia increases A23187-mediated contractile responses in rat thoracic aorta, indicating detrimental effects of short-term hyperglycemia on endothelium-derived contractile factors.
Objective: Vascular tonus has been controlled by several factors secreted from endothelium in physiological conditions.In the present study, the possible changes on endothelium-derived contractile responses in normoglycemic and hyperglycemic conditions in terms of exposure-time dependency has been investigated.Methods: To assess the possible alterations under acute hyperglycemia with different incubation periods on endothelium-derived contractile responses in isolated rat thoracic aorta, A23187-mediated contractile responses were performed in a cumulative manner for isometric tension measurements.Results: Incubation for 3 hours with Krebs solution containing high glucose increased the A23187-mediated contraction of rat thoracic aorta.The A23187induced contraction significantly decreased in response to the same incubation period with normoglycemic conditions and totally abolished with incubation for 6 hours.The possible effects of osmotic pressure induced by high glucose content checked with mannitol.Conclusion: Our results indicated that the A23187-mediated contractile response was increased by acute hyperglycemia.These data has shown the detrimental effects of short term hyperglycemia on endothelium-derived contractile factors.Also the incubation period-related characteristics of A23187-mediated contractile responses as observed in normoglycemic conditions indicate the unstable properties of endothelium-derived contractile factors and/or related signaling pathway(s).
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Işıl Özakça (2018) studied Hyperglycemia (n=33). In vitro high glucose incubation vs. Normoglycemic (11 mM glucose) incubation was evaluated on A23187-mediated contractile response (Emax % of KCl) (p=<0.05). In vitro incubation of rat thoracic aorta with high glucose for 3 hours preserved and increased A23187-mediated contractile responses compared to normoglycemic conditions where responses were significantly depressed.
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