Key result
In vitro treatment of whole blood with the glycoprotein IIb/IIIa inhibitor Integrelin significantly increased the characteristic time of red blood cell aggregate formation from 33.5 to 57.9 seconds.
Why the study?
The study aimed to highlight the problems with implementing optical techniques such as laser tweezers, diffuse light scattering, and laser diffractometry in clinical hemorheological practice.
Optical techniques such as laser tweezers and diffuse light scattering are feasible for assessing the microrheological mechanisms of red blood cell aggregation and deformability.
In vitro GP IIb/IIIa inhibition may slow RBC aggregation; leaves open clinical relevance and requires in vivo validation.
This study aims to highlight the problems with implementing optical techniques (laser tweezers, diffuse light scattering and laser diffractometry) in clinical hemorheological practice. We provide the feasibility of these techniques to assess microrheological effects of various molecular mechanisms affecting RBC aggregation and deformability. In particular, we show that they allow assessment of changes in RBC aggregation in whole blood samples both on the level of single cells and on the level of large ensembles of cells. Application of these methods allows for studying the mechanisms of RBC aggregation because they are sensitive to changes in the medium which surrounds the RBC (i.e., blood plasma, serum or model solutions of blood plasma proteins) and to changes in the cellular properties of RBCs (i.e., effects on the cell membrane due to glycoprotein inhibition).
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Семенов et al. (2022) studied Healthy. Glycoprotein IIb/IIIa inhibitors (Integrelin and Monafram) vs. Control blood samples was evaluated on Characteristic time of aggregate formation (T1/2) for Integrelin (p=<0.05). In vitro treatment of whole blood with the glycoprotein IIb/IIIa inhibitor Integrelin significantly increased the characteristic time of red blood cell aggregate formation from 33.5 to 57.9 seconds.
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