What question did this study set out to answer?

To determine the molecular requirements for outer plaque modification of the spindle pole body and subsequent de novo membrane formation during meiosis II in Saccharomyces cerevisiae.

June 1, 2001Molecular Biology of the CellOpen Access

SPO21Is Required for Meiosis-specific Modification of the Spindle Pole Body in Yeast

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To determine the molecular requirements for outer plaque modification of the spindle pole body and subsequent de novo membrane formation during meiosis II in Saccharomyces cerevisiae.
Examined the subcellular localization of Spo21 protein during meiotic cell division in yeast.
Analyzed structural defects in the outer plaque and prospore membrane assembly in yeast strains lacking SPO21 or harboring reduced SPO21 gene dosage.
Assessed meiotic outer plaque morphology and membrane formation in CNM67 mutant yeast to distinguish general plaque defects from membrane initiation failure.
Spo21p localized to the spindle pole body during meiosis, and deletion of SPO21 led to an abnormal outer plaque structure and total failure of prospore membrane formation.
Reduced dosage of SPO21 restricted competence for membrane assembly to only two of the four meiotic spindle pole bodies.
Mutation of CNM67 disrupted meiotic outer plaque morphology without preventing membrane assembly, demonstrating that outer plaque abnormality alone does not preclude membrane formation.

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Implication

Genetic analysis demonstrates that SPO21 directs spindle pole body remodeling and prospore membrane formation in yeast, indicating its essential role in meiotic packaging.

Cite This Study

Bajgier et al. (2001) studied this question.

synapsesocial.com/papers/6aa46be4f188d5f0710a4693 https://doi.org/10.1091/mbc.12.6.1611

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Implication

Genetic analysis demonstrates that SPO21 directs spindle pole body remodeling and prospore membrane formation in yeast, indicating its essential role in meiotic packaging.

SPO21Is Required for Meiosis-specific Modification of the Spindle Pole Body in Yeast

Key Points

Discussion

Implication

Cite This Study

SPO21Is Required for Meiosis-specific Modification of the Spindle Pole Body in Yeast

Key Points

Discussion

Implication

Cite This Study

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