Key result
Nonradioactive Rb+ efflux assay delivers highest sensitivity and lowest false-hit rate for detecting hERG inhibitors.
Population
hERG potassium channel (in vitro model)
Design
Preclinical
Authors
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May improve preclinical hERG screening efficiency; leaves open confirmation in clinical QT studies.
The nonradioactive Rb+ efflux assay provides the most sensitive and accurate high-throughput method for evaluating hERG channel inhibition compared to dye-based assays.
Tang et al. (2001) studied hERG channel inhibition. Functional hERG channel assay methods was evaluated on Assay sensitivity and false-hit rate. The nonradioactive Rb+ efflux assay was the most sensitive and had the lowest false-hit rate among three evaluated methods for detecting hERG channel inhibitors.
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