Liquid−liquid equilibria of poly(ethylene glycol) (PEG) + potassium phosphate + urea + water aqueous two-phase systems were studied for different PEG molecular weights and pHs. Experimental techniques and analytical methods are described. Equilibrium data were obtained for PEG (1450, 3350, 10000) + potassium phosphate (pH 7 and 9) + urea (6 mass%) + water aqueous two-phase systems at 25 °C. Four tie lines were measured for each system. The partitioning behaviors of lysozyme, catalase, and β-galactosidase were studied in these systems at 25 °C, at pH 7 and 9, and at the urea concentrations 3 and 6 mass %.
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Silva et al. (2001) studied this question.
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