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January 1, 2009Journal of Biomechanical Science and EngineeringOpen Access

ATP Release from Cultured Endothelial Cells and Intercellular Calcium Signaling during Shear Stress Exposure

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Key result

Shear stress triggers endothelial ATP release, driving intercellular calcium communication via purinergic receptors.

  • P<0.05

Population

Cultured endothelial cells (in vitro)

Design

Preclinical

Authors

SKSusumu KUDOKyushu UniversityKHKaoru HOSOEShibaura Institute of TechnologyMHMakoto HOSOBUCHIShibaura Institute of Technology

Discussion

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Implication

No immediate clinical implications; leaves open in vivo relevance of ATP-mediated endothelial calcium signaling.

Key Points

  • To determine whether shear stress induces intercellular calcium communication in endothelial cells and whether this process relies on gap junctions or extracellular ATP release.
  • Cultured endothelial cells were exposed to shear stress loading to evaluate ATP release and track intercellular calcium wave propagation.
  • Local extracellular ATP was manipulated using NPE-caged ATP photolysis, and signaling pathways were probed using purinergic receptor antagonists, ATPase, gap junction inhibitors, and metabolic inhibitors.
  • Shear stress stimulated the release of extracellular ATP from endothelial cells, and localized uncaging of NPE-caged ATP was sufficient to elicit intercellular calcium waves.
  • Intracellular calcium responses under shear stress were markedly inhibited by purinergic receptor blockade, enzymatic ATP degradation via ATPase, and metabolic inhibition using FCCP and rotenone.

Structured PICO

P
Population
In vitro study using cultured bovine aortic endothelial cells to investigate ATP release and intercellular calcium signaling during shear stress exposure.
I
Intervention
Shear stress exposure and NPE-caged ATP stimulation, with or without chemical inhibitors (purinergic receptor blocker, ATPase, FCCP, rotenone)
O
Outcome
ATP release and intercellular Ca2+-waves ([Ca2+]i responses)surrogate

Main Result

Absolute Event Rate: 10% vs 0%

p-value: p=<0.05

Intercellular calcium communication in endothelial cells exposed to shear stress is mediated by ATP release, providing mechanistic insight into endothelial mechanotransduction.

Limitations

  • Could not quantitatively analyze the relationship between ATP release from a single BAEC and [Ca2+]i signals during shear stress exposure.
  • Impossible to locate the precise endothelial cell that released the ATP in response to shear stress exposure.

Cite This Study

KUDO et al. (2009) studied Cultured Endothelial Cells. Shear stress vs. Baseline (no shear stress) was evaluated on ATP release after 20 seconds of shear stress exposure (p=<0.05). Cultured bovine aortic endothelial cells subjected to 2 Pa of shear stress released ATP (up to 10 pM), mediating intercellular calcium communication that was inhibited by purinergic receptor blockers.

synapsesocial.com/papers/6aa4d4242fdbcb5be994dff1https://doi.org/10.1299/jbse.4.274
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Flow-induced changes in Ca2+ signaling of vascular endothelial cells: effect of shear stress and ATP1991 · 175 citations
  2. 2Inhibition of ecto‐ATPase by PPADS, suramin and reactive blue in endothelial cells, C6glioma cells and RAW 264.7 macrophages1996 · 94 citations
  3. 3Flow modulation of agonist (ATP)-response (Ca2+) coupling in vascular endothelial cells1991 · 189 citations
  4. 4Mechanotransduction and endothelial cell homeostasis: the wisdom of the cell2006 · 918 citations