What question did this study set out to answer?

To quantitatively analyze the protein-nucleic acid interactions and structural intermediates governing the assembly of the Escherichia coli 30S ribosomal subunit.

September 15, 1991Proceedings of the National Academy of SciencesOpen Access

Assembly of the Escherichia coli 30S ribosomal subunit reveals protein-dependent folding of the 16S rRNA domains.

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To quantitatively analyze the protein-nucleic acid interactions and structural intermediates governing the assembly of the Escherichia coli 30S ribosomal subunit.
Reconstituted ribonucleoprotein core particles in vitro through the sequential addition of ribosomal proteins to 16S rRNA.
Characterized particle morphology, mass, and radii of gyration using high-resolution scanning transmission electron microscopy.
Assessed the functional activity of fully reconstituted 30S subunits using polyphenylalanine synthesis assays.
Binding of the initial 12 ribosomal proteins produced intermediate core particles with up to three distinct mass centers matching the 5', central, and 3' domains of 16S rRNA.
Late-assembling ribosomal proteins prompted two of the three RNA domains to merge, generating the foundational architecture of the native 30S subunit.
Complete protein addition yielded structurally resolved particles that exhibited full functional activity in polyphenylalanine synthesis assays.

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Implication

In vitro structural study reveals protein-dependent domain folding during 30S ribosomal subunit assembly in Escherichia coli, highlighting distinct ribonucleoprotein intermediate core particles.

Cite This Study

Mandiyan et al. (1991) studied this question.

synapsesocial.com/papers/6aa4d4c572bda1c777aa20ef https://doi.org/10.1073/pnas.88.18.8174

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Implication

In vitro structural study reveals protein-dependent domain folding during 30S ribosomal subunit assembly in Escherichia coli, highlighting distinct ribonucleoprotein intermediate core particles.

Assembly of the Escherichia coli 30S ribosomal subunit reveals protein-dependent folding of the 16S rRNA domains.

Key Points

Discussion

Implication

Cite This Study

Assembly of the Escherichia coli 30S ribosomal subunit reveals protein-dependent folding of the 16S rRNA domains.

Key Points

Discussion

Implication

Cite This Study

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