The Warthin-Starry1 procedure, according to Kerr2, appears to be -one of the more reliable methods for demonstration of spirochaetes in single paraffin sections. However, the adjustment of the pH of the distilled water used to pH 4.4 with citric acid necessitates the use of indicator each time, and, further, the citric acid stock solution has to be made fresh at frequent intervals because of its susceptibility to mold growth.
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Faulkner et al. (1945) studied this question.
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