Key result
Low-dose doxycycline controls allosteric hammerhead ribozyme activity as a molecular switch for conditional gene expression.
An allosteric hammerhead ribozyme regulated by doxycycline was developed, which may serve as a tool for tailored conditional gene expression systems.
May enable tunable gene expression in models; leaves open translation to cardiovascular therapies.
Low concentrations of the antibiotic doxycycline are sufficient to control the activity of an allosteric hammerhead ribozyme (shown schematically). This was obtained by allosteric selection of an RNA library, which was fused to helix II of the ribozyme. Such molecular switches may serve as a valuable tool for the development of tailored conditional gene expression systems that can be controlled by the presence or absence of any kind of small molecule.
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Piganeau et al. (2000) studied this question. Doxycycline was evaluated on Control of the activity of an allosteric hammerhead ribozyme. Low concentrations of doxycycline were sufficient to control the activity of an allosteric hammerhead ribozyme, demonstrating a molecular switch for conditional gene expression.
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