Key result
Inserting short foreign nucleotide sequences upstream of rVP1 increases sapovirus VLP yield in insect cells.
Inserting short foreign nucleotide sequences upstream of the rVP1 start codon enhances the expression of sapovirus recombinant capsid protein in insect cells.
May aid sapovirus VLP production; leaves open translation beyond insect cells.
Human sapovirus (SaV) is uncultivable, but expression of the recombinant capsid protein (rVP1) in insect cells results in the formation of virus-like particles (VLPs) that are morphologically similar to the native viruses. However, the SaV rVP1 expression levels are considerably low. We have found that inclusions of short foreign nucleotide sequences inserted directly upstream from the predicted rVP1 AUG start codon lead to increased yield of VLPs. This method allowed us to express a SaV rVP1, which could not have been expressed to measurable or practical levels otherwise.
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Hansman et al. (2006) studied Human sapovirus (SaV). Insertion of short foreign nucleotide sequences upstream from the rVP1 AUG start codon was evaluated on Yield of virus-like particles (VLPs). Insertion of short foreign nucleotide sequences directly upstream from the predicted rVP1 AUG start codon led to increased yield of sapovirus virus-like particles in insect cells.
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