Brefeldin A, a specific inhibitor of intracellular translocation of vesicular stomatitis virus G protein: Intracellular accumulation of high-mannose type G protein and inhibition of its cell surface expression.

Synthesis and intracellular translocation of glycoproteins destined to be exported extracellularly or sequestered on cell surfaces have attracted much interest recently.Virus envelope glycoproteins have been the subject of intense studies because, in many cases, cellular macromolecular synthesis is shut off after virus infection and thus the subsequent synthesis and fate of virus components are faciliated.^Thepolypeptide moiety of vesicular stomatitis virus (VSV) G protein is synthesized on membrane-boundpolysomes and the oligosaccharide moiety is transferred en