BMAL1 may be involved in angiogenesis and peritumoral cerebral edema of human glioma by regulating VEGF and ANG2

Figures

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  Figure 1. Immunohistochemistry analysis of BMAL1, HIF-1a, ANG1, ANG2, and VEGF expression in glioma and normal tissues with different pathological grades.

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  Figure 2. Expression analysis of BMAL1 and CD34. (A) ANOVA test was used for comparisons the expression of BMAL1 in glioma and normal tissues with different pathological grades. (B) A set of representative graphs displaying the number of microvessels in gliomas by labeling CD34. (C) Two-sample t-test was used for comparisons between the two groups.*p < 0.05, **p < 0.01. ***p < 0.001.

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  Figure 3. MR images of clinical patients showed the relationship between peritumoral brain edema and BMAL1 expression. (A) A set of representative graphs displaying MR images of brain edema. The red dotted line is the solid part of the tumor, and the yellow dotted line is the area of brain edema around the tumor. Patients with BMAL1 (+) had obvious peritumoral edema. (B) The EI was compared between the BAML1 (+) expression group (n = 59) and the BAML1 (−) expression group (n = 20). (C) BMAL1 expression levels in the mild edema group (n = 5), moderate edema group (n = 15), and severe edema group (n = 39) were assessed. *p < 0.05, **p < 0.01. ***p < 0.001.

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  Figure 4. The regulatory pathway of BMAL1 on proangiogenic factors was assessed by western blot. (A) A set of representative graphs displaying the regulatory pathway of BMAL1 in glioma cells. (B) Two-sample t-test was used for comparisons between the two groups. *p < 0.05, **p < 0.01. ***p < 0.001.