Isolation and Characterization of Mutants in Plant Cell Culture

INTRODUCTION . 519 NOMENCLATURE 520 METHODS FOR THE ISOLATION OF VARIANT CELL LINES 521 BASIS OF PHENOTYPIC VARIATION IN SOMATIC CELLS 522 St�ies of SeJ;�1 Transmission to Confirm Mutation 522 Epigenetic Varrants.. . . ..... ... .. . . . ..... . . .. ...... . . .. ........ 525 CHARACTERIZATION OF DOMINANCE-RECESSIVITY RELATIONSHIPS BY CELL FUSION 526 MUTANTS AND VARIANTS 526 Auxotrophic Lines , . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .... . . . . . . . . . . .. . . . . . . . . . . 526 Chlorate Resistance-Nitrate Reductase Deficiency 527 Resistance to Amino Acids and Amino Acid Analogs 528 Res!s�an�e to !ucleic Acid Base Analogs 530 AntibIOtIC ReSistance 530 Developmental Variants 532 Herbicide Resistance 532 Salt and Water Stress Tolerance 533 Disease Tolerance 534 SOMACLONAL VARIATION . ... . . . . . . .. . . . . . . . . . . .. .... . . . . . . . . ....... . . . . . . . 535 CONCLUSIONS AND REMARKS 536