The Immune Efficacy of Inactivated Pseudorabies Vaccine Prepared from FJ-2012ΔgE/gI Strain

Figures

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  Figure 1. Development of anti-gB (A) and gE (B) antibodies with ELISA. (A) gB-specific antibody levels: a sample with S/N ratios ≤ 0.60 were classified as positive for gB antibodies. (B) gE-specific antibody levels: a sample with S/N ratios ≤ 0.60 were classified as positive for gE antibodies. The “*” mark in the group top right indicates an inactivated vaccine.

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  Figure 2. The neutralizing antibody titers of immunized piglets (n = 5) in a vaccination group. At 21, 35, and 42 days post-immunization, serum samples were collected and tested by SVNT. Statistical analysis was performed using GraphPad Prism version 6.0 for Windows (Dr. Harvey Motulsky, GraphPad Software, CA, USA). Pairwise comparisons of neutralizing antibody titers between different groups were performed by one-way ANOVA, followed by Tukey’s multiple comparison test. Significant differences (p < 0.01) are marked by **; (p < 0.05) by *; and no significant difference by ns.

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  Figure 3. Protection efficacy of different vaccines against PRV FJ-2012 challenge in piglets. (A) Rectal temperature of piglets after challenging with FJ-2012. (B) Clinical scores with mean values and standard deviation. (C) Percentage survival curve of piglets vaccinated with a different vaccine. Survival is presented as a Kaplan–Meier plot (n = 5 per group). The “*” mark in the group top right indicates an inactivated vaccine.

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  Figure 4. Histopathological findings of piglet tissues after PRV challenge. The letters A to E refer to the lung, kidney, tonsil, brain, and spleen, respectively. The numbers 1 to 6 represent the FJ-2012-GEL*, FJ-2012-VG*, vPRV*, K61, unimmunized challenged, and control groups, respectively. Hematoxylin and eosin staining (H.E.), Magnification, 200×. The “*” mark in the group top right indicates an inactivated vaccine.

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  Figure 5. Viral load in different tissue samples from piglets after challenge. Viral genome numbers are presented as mean ± SD as measured by one-way repeated measurement analysis of variance (ANOVA) and least significant difference (LSD); the uppercase letters on the group bar indicate that the difference was extremely significant (p < 0.01); lowercase letters indicate a significant difference (p < 0.05). The “*” mark in the group top right indicates an inactivated vaccine.