Effect of Intravenous Glucose on Concentration of Insulin in Maternal and Umbilical-cord Plasma

using a ferricyanide method (Hoffman, 1937), within 36 hours.This period of storage was accepted, since, when the same sample was estimated repeatedly at different times (0-36 hours), any change in blood-sugar concentration was less than the error of the method.For insulin, blood was collected in dry heparinized bottles and centrifuged immediately ; the separated plasma was stored at -10° C. until the insulin concentration was estimated by method C of the immunoassay described by Hales and Randle (1963).