Dimethyl sulfoxide (DMSO) was evaluated, in terms of motility, as a freeze-thaw protective agent with human spermatozoa. DMSO was found to be equal to glycerol in this protective capacity but proved more toxic during storage at 22°C both before freezing and after thawing. An interaction of protective and toxic effects was noted in a comparison of 5, 10, 15 and 20% DMSO which suggested the 10% level as the most favorable. On the basis of findings, DMSO is not recommended as a substitute for glycerol in the preservation of human spermatozoa by freezing with the methods described.
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J.K. Sherman (1964) studied this question.