What question did this study set out to answer?

To determine the contribution of mast cells and leukotriene mediators to immune complex-induced tissue injury using a murine reverse Arthus reaction model.

September 1, 1991Journal of Clinical InvestigationOpen Access

Augmentation of reverse arthus reaction by mast cells in mice.

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Authors

YZY ZhangWashington University in St. Louis BRB F RamosWashington University in St. Louis BJBarbara A. JakschikWashington University in St. Louis

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Implication

Animal study demonstrates that mast cells and leukotrienes amplify immune complex-mediated tissue injury in mice, indicating a critical role in Arthus-type inflammation.

Key Points

To determine the contribution of mast cells and leukotriene mediators to immune complex-induced tissue injury using a murine reverse Arthus reaction model.
Induced cutaneous reverse Arthus reactions in mast cell-deficient WBB6F1-W/Wv mice, congenic WBB6F1-+/+ controls, and mast cell-reconstituted WBB6F1-W/Wv mice.
Assessed tissue edema, hemorrhage, neutrophil influx in serial skin sections, and mast cell activation by avidin-FITC granule staining.
Treated control and mast cell-deficient mice with the 5-lipoxygenase inhibitor A-63162 to measure leukotriene-dependent inflammation.
Mast cell-deficient mice exhibited a 60% reduction in neutrophil influx (40% of control levels), a 40% reduction in edema (60% of control levels), and significantly reduced hemorrhage compared to congenic controls.
Local mast cell reconstitution in deficient mice completely restored neutrophil influx, edema, and hemorrhage to levels equivalent to wild-type controls, with a 70% decrease in avidin-FITC fluorescence confirming mast cell release.
The 5-lipoxygenase inhibitor A-63162 significantly reduced neutrophil accumulation by 40%, edema by 60%, and hemorrhage in controls and reconstituted mice, but produced no effect in mast cell-deficient mice.

Cite This Study

Zhang et al. (1991) studied this question.

synapsesocial.com/papers/6aa5bdd59feada129378e824 https://doi.org/10.1172/jci115385

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Implication

Animal study demonstrates that mast cells and leukotrienes amplify immune complex-mediated tissue injury in mice, indicating a critical role in Arthus-type inflammation.

Augmentation of reverse arthus reaction by mast cells in mice.

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Implication

Key Points

Cite This Study

Augmentation of reverse arthus reaction by mast cells in mice.

Discussion

Member takes

Implication

Key Points

Cite This Study

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