What is the clinical evidence from this study?

Study design: Other. Population: Cultured neonatal Wistar rat cardiomyocytes. Intervention: Cyclic mechanical stretch vs. Control cells without stretch. Primary outcome: TLR4 protein expression (3.1-fold increase, p=<0.001).

What does this research mean for the field?

Cyclic mechanical stretch enhances TLR4 expression in cultured rat neonatal cardiomyocytes through activation of the p38 MAP kinase and NF-κB pathways, increasing monocyte adherence. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

March 5, 2010Journal of Biomedical ScienceOpen Access

Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-κB pathway

Q: What are the key findings of this study?

Cyclic mechanical stretch significantly increased TLR4 protein expression by 3.1-fold in cultured rat neonatal cardiomyocytes, mediated through activation of p38 MAP kinase and NF-κB pathways.

Q: What does this research mean for the field?

Cyclic mechanical stretch enhances TLR4 expression in cultured rat neonatal cardiomyocytes through activation of the p38 MAP kinase and NF-κB pathways, increasing monocyte adherence. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

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Key result

Cyclic mechanical stretch increases TLR4 expression ~3-fold in cardiomyocytes via p38 MAPK and NF-κB.

3.1-fold increase
P<0.001

Population

Cultured neonatal Wistar rat cardiomyocytes (from rats aged 2-3 days old)

Comparison

Cyclic mechanical stretch for 2 to 24 hours vs Control cardiomyocytes without stretch

Design

Preclinical

Follow-up

up to 24 hours

Authors

KSKou‐Gi ShyuFu Jen Catholic University BWBao-Wei WangFu Jen Catholic University CLChiu-Mei LinMemorial Hospital

Discussion

Member takes

Implication

May link mechanical overload to cardiomyocyte inflammation; leaves open TLR4 targeting in heart failure.

Structured PICO

Population

In vitro study of cultured neonatal Wistar rat cardiomyocytes subjected to cyclic mechanical stretch.

Intervention

Cyclic mechanical stretch (vacuum to 20% of maximum elongation at 60 cycles/min) for 2 to 24 hours

Comparator

Control cardiomyocytes without stretch

Outcome

TLR4 protein and mRNA expressionsurrogate

Main Result

Effect estimate: 3.1-fold increase

Absolute Event Rate: 3.1% vs 1%

p-value: p=<0.001

Cyclic mechanical stretch upregulates TLR4 expression in cardiomyocytes via p38 MAP kinase and NF-κB pathways, which increases monocyte adherence and may contribute to inflammation in mechanically overloaded hearts.

Cite This Study

Shyu et al. (2010) studied Cultured neonatal Wistar rat cardiomyocytes. Cyclic mechanical stretch vs. Control cells without stretch was evaluated on TLR4 protein expression (3.1-fold increase, p=<0.001). Cyclic mechanical stretch significantly increased TLR4 protein expression by 3.1-fold in cultured rat neonatal cardiomyocytes, mediated through activation of p38 MAP kinase and NF-κB pathways.

synapsesocial.com/papers/6aa5be8604ea443b843a4f6f https://doi.org/10.1186/1423-0127-17-15

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Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-κB pathway

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-κB pathway

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider