Key result
A direct colorimetric LAMP assay demonstrated sensitivity, specificity, and limit of detection comparable to a commercial real-time PCR kit for detecting African swine fever virus.
Why the study?
Recommended real-time PCR for African swine fever requires DNA extraction and high-cost, non-portable equipment, necessitating a rapid, portable diagnostic method to control outbreaks.
Does a direct colorimetric LAMP assay provide comparable diagnostic performance to real-time PCR for detecting African swine fever virus in crude serum samples from domestic pigs?
Does a direct colorimetric LAMP assay provide comparable diagnostic performance to real-time PCR for detecting African swine fever virus in crude serum samples from domestic pigs?
A direct colorimetric LAMP assay offers a rapid, extraction-free, and equipment-light alternative to real-time PCR for in-field diagnosis of African swine fever virus.
May enable field ASFV screening in pigs; leaves open validation for routine or regulatory use.
African swine fever (ASF) is a highly infectious viral disease with high mortality. The most recent ASF outbreak in Vietnam began in 2019, posing a threat to spread to the neighbouring Asian countries. Without a commercial vaccine or efficient chemotherapeutics, rapid diagnosis and necessary biosecurity procedures are required to control the disease. While the diagnostic method of ASF recommended by the World Organization of Animal Health is real-time PCR, the ideal diagnosis procedure including master mix setup, template extraction and a high-cost qPCR equipment for many samples being tested simultaneously is not portable. In this study, a colorimetric loop-mediated isothermal amplification (LAMP) assay was modified and evaluated for ASF virus detection using crude serum samples collected from domestic pigs in Vietnam during the 2019 outbreak. The LAMP results can be readily visualized to the naked eye within 30 min without the requirement of DNA extraction and sophisticated equipment. The sensitivity, specificity and limit of detection of direct colorimetric LAMP assay were comparable to a commercial diagnostic real-time PCR kit. Results strongly indicate that the adapted colorimetric LAMP assay has a remarkable potential for the in-field diagnosis of ASF.
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Tran et al. (2020) studied African swine fever (ASF). Colorimetric loop-mediated isothermal amplification (LAMP) assay vs. Commercial diagnostic real-time PCR kit was evaluated on Sensitivity, specificity and limit of detection. A direct colorimetric LAMP assay demonstrated sensitivity, specificity, and limit of detection comparable to a commercial real-time PCR kit for detecting African swine fever virus.
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