Key result
The colorimetric LAMP assay demonstrated 100% sensitivity and accuracy for detecting African swine fever virus in crude pig serum samples, matching the performance of commercial real-time PCR.
Why the study?
African Swine Fever is highly infectious with high mortality, and while real-time PCR is recommended, it is not suitable for in-field detection.
Does a colorimetric LAMP assay accurately detect African swine fever virus in crude serum samples compared to real-time PCR?
Does a colorimetric LAMP assay accurately detect African swine fever virus in crude serum samples compared to real-time PCR?
The developed colorimetric LAMP assay provides a rapid, sensitive, and equipment-free method for in-field diagnosis of African swine fever virus.
Answers are grounded in this paper and cite their sources.
May enable rapid ASFV testing in field settings; leaves open prospective validation before clinical adoption.
African Swine Fever (ASF) is a highly infectious viral disease with high mortality. The most recent ASF outbreak in Vietnam occurred in 2019, posing a threat to spread to the neighboring Asian countries. Without a commercial vaccine or efficient chemotherapeutics successfully developed, rapid diagnosis and necessary biosecurity procedures are required to control the disease. While the diagnosis method of ASF recommended by the World Organization of Animal Health is real-time PCR, it is not suitable for in-field detection of the disease. In this study, a colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay was developed and evaluated for ASF virus detection using crude serum samples collected from domestic pigs in Vietnam during the 2019 outbreak. The LAMP results can be readily visualized to naked eyes within 30 minutes without the requirement of DNA extraction and sophisticated equipment. The sensitivity, specificity, and limit of detection of colorimetric LAMP assay were comparable to a commercial diagnostic real-time PCR kit. Results strongly indicate that the developed colorimetric LAMP assay is highly recommended for the in-field diagnosis of ASF.
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Tran et al. (2020) studied African Swine Fever (n=95). Colorimetric LAMP assay vs. Commercial real-time PCR kit was evaluated on Diagnostic sensitivity and accuracy. The colorimetric LAMP assay demonstrated 100% sensitivity and accuracy for detecting African swine fever virus in crude pig serum samples, matching the performance of commercial real-time PCR.
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