What question did this study set out to answer?

To determine the role of the receptor tyrosine phosphatase GLEPP1 (Ptpro) in maintaining podocyte morphology and regulating glomerular filtration and blood pressure.

November 15, 2000Journal of Clinical InvestigationOpen Access

Altered podocyte structure in GLEPP1 (Ptpro)-deficient mice associated with hypertension and low glomerular filtration rate

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Authors

BWBryan L. WharramJohns Hopkins University MGMeera GoyalUniversity of Michigan PGPatrick J. GillespieIndiana University School of Medicine

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Implication

Experimental mouse study demonstrates altered podocyte architecture and reduced filtration rate in Ptpro-deficient mice, indicating a regulatory role in glomerular pressure dynamics.

Key Points

To determine the role of the receptor tyrosine phosphatase GLEPP1 (Ptpro) in maintaining podocyte morphology and regulating glomerular filtration and blood pressure.
Generated Ptpro-deficient (Ptpro-/-) mice through targeted exon disruption in embryonic stem cells.
Evaluated renal histology and cytoskeletal distribution using light microscopy, scanning electron microscopy, transmission electron microscopy, and vimentin localization.
Assessed glomerular filtration rate, urine albumin excretion, glomerular nephrin content, and post-nephrectomy blood pressure versus wild-type littermates.
Ptpro(-/-) mice developed blunted, widened foot processes with an amoeboid rather than normal octopoid morphology, alongside altered intermediate cytoskeletal vimentin distribution.
Glomerular filtration rate and glomerular nephrin levels were reduced in Ptpro(-/-) mice, while urine albumin excretion remained comparable to wild-type controls.
Ptpro(-/-) mice exhibited significantly elevated blood pressure compared to wild-type littermates following the surgical reduction of renal mass.

Cite This Study

Wharram et al. (2000) studied this question.

synapsesocial.com/papers/6aa618f486a9fcb249922ff6 https://doi.org/10.1172/jci7236

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Implication

Experimental mouse study demonstrates altered podocyte architecture and reduced filtration rate in Ptpro-deficient mice, indicating a regulatory role in glomerular pressure dynamics.

Altered podocyte structure in GLEPP1 (Ptpro)-deficient mice associated with hypertension and low glomerular filtration rate

Discussion

Member takes

Implication

Key Points

Cite This Study

Altered podocyte structure in GLEPP1 (Ptpro)-deficient mice associated with hypertension and low glomerular filtration rate

Discussion

Member takes

Implication

Key Points

Cite This Study

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