Key result
Human placenta cDNA fragment encoding preproendothelin-1 and its carboxyl terminal mature precursor was successfully expressed in E. coli and characterized by immunoassay and Western blot.
The study demonstrates the successful synthesis and characterization of human endothelin-1 precursors in E. coli, providing a method for producing this potent vasoconstrictor for further research.
Enables bacterial production of endothelin-1 precursors for lab use; leaves open any clinical cardiovascular applications.
Endothelin, the most potent vasoconstrictor found in nature, is thought to be important in the regulation of blood pressure and/or local blood distribution. Human placenta cDNA fragment encoding preproendothelin-1 (preproET-1) and its carboxyl terminal mature precursor (C-matured precursor) was expressed in E. coli. These products were characterized by both enzyme immunoassay and Western blot analysis.
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Watanabe et al. (1989) studied this question. Expression of human placenta cDNA fragment encoding preproendothelin-1 was evaluated on Characterization of synthesized products. Human placenta cDNA fragment encoding preproendothelin-1 and its carboxyl terminal mature precursor was successfully expressed in E. coli and characterized by immunoassay and Western blot.
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