Isolation of the pathogen and inoculum preparationBanana fruits showing anthracnose disease symptoms were collected and used for isolation of Colletotrichum musae.Pure culture was maintained on PDA medium.Inoculum was prepared by dislodging the surface of 10 days old culture with sterile distilled water and adjusted to 105 ml -1 using a hemacytometer. In vitro assay of plant extractsPaper disc method: The method as described by [9] was used.
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mare Ayalew Kebede Woldetsadik (2014) studied this question.
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