What is the clinical evidence from this study?

Study design: Other. Population: Respiratory Syncytial Virus. Primary outcome: Coupled translation of the second open reading frame (ORF) of the RSV M2 transcript.

What does this research mean for the field?

Coupled translation of the respiratory syncytial virus M2-2 open reading frame requires structured upstream sequences located approximately 150 nucleotides upstream of the start codon in addition to the ORF overlap region. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

March 23, 2005Journal of Biological ChemistryOpen Access

Coupled Translation of the Respiratory Syncytial Virus M2 Open Reading Frames Requires Upstream Sequences

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Key result

Mutating upstream secondary structures ~150 nucleotides before ORF2 impairs RSV M2 coupled translation.

Population

Respiratory syncytial virus M2 transcript

Comparison

Mutation of sequences upstream of the ORF2… vs Wild-type/unmutated sequences

Design

Preclinical

Authors

PGPhillip S. GouldCoventry University AEAndrew J. EastonHealth Economics and Outcomes Research (United Kingdom)

Discussion

Member takes

Implication

May inform RSV antiviral design targeting M2 translation; leaves open in vivo relevance and clinical translation.

Structured PICO

Population

Respiratory syncytial virus M2 transcript

Intervention

Mutation of sequences upstream of the ORF2 initiation codons to remove predicted areas of base pairing

Comparator

Wild-type/unmutated sequences

Outcome

Efficiency of coupled translationsurrogate

Upstream sequences with significant secondary structure act in conjunction with the M2-1/M2-2 overlap region to promote coupled translation of the RSV M2 transcript.

Cite This Study

Gould et al. (2005) studied Respiratory Syncytial Virus. Coupled translation of the respiratory syncytial virus M2 open reading frames requires upstream sequences with significant secondary structure located approximately 150 nucleotides upstream of ORF2.

synapsesocial.com/papers/6aa65cce50dab972bddd6505 https://doi.org/10.1074/jbc.m502276200

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March 23, 2005Journal of Biological ChemistryOpen Access

Coupled Translation of the Respiratory Syncytial Virus M2 Open Reading Frames Requires Upstream Sequences

View Full Paper

Ask AI

Bookmark

Key result

Mutating upstream secondary structures ~150 nucleotides before ORF2 impairs RSV M2 coupled translation.

Population

Respiratory syncytial virus M2 transcript

Comparison

Mutation of sequences upstream of the ORF2… vs Wild-type/unmutated sequences

Design

Preclinical

Authors

PGPhillip S. GouldCoventry University AEAndrew J. EastonHealth Economics and Outcomes Research (United Kingdom)

Discussion

Member takes

Implication

May inform RSV antiviral design targeting M2 translation; leaves open in vivo relevance and clinical translation.

Structured PICO

Population

Respiratory syncytial virus M2 transcript

Intervention

Mutation of sequences upstream of the ORF2 initiation codons to remove predicted areas of base pairing

Comparator

Wild-type/unmutated sequences

Outcome

Efficiency of coupled translationsurrogate

Upstream sequences with significant secondary structure act in conjunction with the M2-1/M2-2 overlap region to promote coupled translation of the RSV M2 transcript.

Cite This Study

synapsesocial.com/papers/6aa65cce50dab972bddd6505 https://doi.org/10.1074/jbc.m502276200

Also Consider

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