Key result
High extracellular LDL decreases heparan sulfate proteoglycan content in cultured aortic endothelial cells.
High concentrations of LDL decrease basement membrane-associated heparan sulfate proteoglycan in cultured porcine aortic endothelial cells, providing insight into LDL-mediated endothelial dysfunction.
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High LDL may impair endothelial matrix in animal models; leaves open relevance to human atherosclerosis without in vivo confirmation.
The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoassay. Low extracellular LDL-levels increase, high extracellular LDL-levels decrease the HSPG content of PAEC. Fibronectin synthesis was only slightly affected while proliferation and metabolic activity as assessed by lactate production were constant. Insulin or high extracellular glucose did not influence the effect of LDL on basement membrane components.
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Olgemöller et al. (1990) studied this question. High concentrations of low density lipoprotein (LDL) vs. Low extracellular LDL-levels was evaluated on Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin content. High extracellular LDL concentrations decreased the heparan sulfate proteoglycan content of cultured porcine aortic endothelial cells, while fibronectin synthesis was only slightly affected.
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