The Culture of the Rumen Organism Eadie's Oval in vitro

SUMMARY Clone cultures of two strains of the rumen organism Eadie’s Oval were established in vitro. Both strains were maintained anaerobically at 38 to 40° in liquid culture in the presence of bacteria, rumen fluid, a reducing agent and a CO2 gas phase. There was no growth in sterile or solid media. Growth and storage of glycogen occurred when each strain was incubated with one of a small range of simple carbohydrates; amino acids, fatty acids, proteins and polysaccharides did not support growth or glycogen synthesis. Neither strain survived freezing, but cultures were viable after two weeks at 4°; incubation at 44° resulted in no growth or the death of cultures.