Key result
Microinjected fluorescent actin and tropomyosin incorporate into known myofibrillar locations in living cardiac myocytes.
Population
Cultured cardiac myocytes and skeletal muscle myotubes
Comparison
Microinjection of fluorescently labeled actin… vs Isolated myofibrils
Design
Preclinical
Authors
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Highlights dynamic protein exchange in living myocytes; leaves open relevance to human cardiac remodeling.
Myofibrils in living cardiac and skeletal muscle cells are dynamic structures capable of exchanging actin and tropomyosin molecules, unlike isolated myofibrils.
Dome et al. (1988) studied Cultured cardiac myocytes and skeletal muscle myotubes. Microinjection of fluorescently labeled actin and tropomyosin vs. Isolated myofibrils was evaluated on Incorporation patterns of labeled actin and tropomyosin. Microinjection of fluorescently labeled actin and tropomyosin into living cardiac myocytes and skeletal myotubes resulted in incorporation patterns matching their known myofibrillar localization.
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