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April 9, 2009Journal of Clinical MicrobiologyOpen Access

Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic Escherichia coli

Francesca Barletta Theresa J. Ochoa Lia Ecker Ana M. Gil Claudio F. Lanata Thomas G. Cleary

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Abstract

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

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Barletta et al. (2009) studied this question.

synapsesocial.com/papers/6aa6bee0e46a8f6037922ca5 https://doi.org/10.1128/jcm.00608-09

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