Answers are grounded in this paper and cite their sources.
We report the development of automated flourometric immunoassay for the detection of Escherichia coli O157:H7, using antibody‐directed liposomes (immunoliposomes) encapsulating fluorophore as an analytical reagent. Thiolated antibodies (anti‐ E. coli O157:H7) were coupled to malemide‐tagged liposomes encapsulating dye. To automate the assay, a fluorescence plate reader was included in the assay system to detect fluorophore released from lysed liposomes in a microplate. The detection limit of the current assay with pure cultures of the serotype was about 10 4 colony‐forming units (CFU)/mL. The assay can detect E. coli O157 in ground beef samples inoculated with as few as 0.8 CFU/mL after a 12‐h enrichment. These results demonstrate the feasibility of using fluorophore‐encapsulated immunoliposomes in a microtiter plate for the rapid and automated detection of molecules with multivalent antigenic sites.
No takes yet. Share an insight, caveat, or question.
Park et al. (2004) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: