Traditional Chinese Medicine ShenZhuGuanXin Granules Mitigate Cardiac Dysfunction and Promote Myocardium Angiogenesis in Myocardial Infarction Rats by Upregulating PECAM‐1/CD31 and VEGF Expression

Figures

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  Figure 1. Thin-layer chromatography (TLC) was used for qualitative analysis part of active ingredients of SG, which was detected containing pseuoginsenoside F11, ginsenoside Rg1, ginsenoside Re, and ginsenoside Rb1. Solvent was used as negative control. Positive control is a mixture solution, containing four standard substances: pseuoginsenoside F11, ginsenoside Rg1, ginsenoside Re, and ginsenoside Rb1. (a) and (b) were inspected under fluorescence and ultraviolet, respectively.

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  Figure 2. High performance liquid chromatography (HPLC) was selected for identification of the major chemical compounds of SG. Fingerprint chromatogram (a) is the peaks of four standards. Same peaks were identified in (b) (SG sample). 1, notoginsenoside R1; 2, ginsenoside Rg1; 3, ginsenoside Re; 4, ginsenoside Rb1.

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  Figure 3. Infarction size assessment by Masson's trichrome staining. (a) Representative myocardial sections are shown. (b) SG-M and SG-H treatment significantly reduce the infarction size compared with MI group. Each value represents the mean ± SD of nine rats. ∗P < 0.05 versus sham group, #P < 0.05 versus MI group, ∆P < 0.05 versus DSDW group, ▲P < 0.05 versus SG-L group.

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  Figure 4. SG and CDDP treatment increased microvessel density (MVD) after MI. (a) MVD in the myocardial infarction border zone by HE staining; (b) quantitative analysis. Each value represents the mean ± SD of nine rats. ∗P < 0.05 versus sham group, #P < 0.05 versus MI group, ∆P < 0.05 versus DSDW group, ▲P < 0.05 versus SG-L and SG-M group. Magnification was 200x.

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  Figure 5. Immunohistochemical staining of PECAM-1/CD31 (a) and VEGF (b). (a) The expression of PECAM-1/CD31 was increased significantly in SG-L, SG-M, SG-H, and CDDP group compared with MI group; besides, (b) SG-M, SG-H, and CDDP treatment significantly increased the expression of VEGF compared with MI group. Each value represents the mean ± SD of nine rats. ∗P < 0.05 versus sham group, #P < 0.05 versus MI group, ∆P < 0.05 versus DSDW group, ▲P < 0.05 versus SG-L and SG-M group. Magnification was 200x.