Development of CRISPR-Cas9 Multiple Editing System for Genetic Improvement of Rice

The CRISPR-Cas9 multiple editing system has become an important tool in the field of rice genetic improvement. This review aims to outline the principles and applications of the system, emphasizing its cutting-edge position in rice breeding. The advantage of a multiple editing system is that it can simultaneously edit multiple loci to achieve precise improvement of rice yield, resistance, and quality traits. This review also discusses in detail the working principle, development process, and widespread application of multiple editing systems, briefly introduces CRISPR-Cas9 technology, explains how multiple editing systems can achieve efficient multi gene editing, and delves into the specific applications of multiple editing systems in rice genetic improvement, including increasing yield, increasing resistance, and improving quality. These applications have enriched the genetic resources of rice and provided new avenues for food security and sustainable agricultural development.