Single-stranded DNA extracted from virions of the originally described tomato yellow leaf curl virus (TYLCV) served as a template for in vitro synthesis of its double-stranded form. Analysis with different restriction enzymes yielded fragments with an overall size of approximately 5.6 kb, twice the suggested virus genome unit size. Based on restriction analysis and hybridization tests, it is suggested that the first described culture of TYLCV consists of two viral DNA populations reflecting some slight sequence differences. Agroinoculation of TYLCV hosts with one of the cloned viral DNA populations resulted in systemic infection, but symptoms on some tomato varieties were significantly milder than those induced by the native virus [...]
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Y. Antignus (1994) studied this question.