Key result
High pressure and pulsatile flow perfusion for 3 hours caused morphological changes and decreased eNOS expression in human saphenous veins from diabetic patients.
Why the study?
Does elevated perfusion pressure and pulsatile flow affect morphology and NOS expression in human saphenous veins from diabetic vs non-diabetic patients?
Does elevated perfusion pressure and pulsatile flow affect morphology and NOS expression in human saphenous veins from diabetic vs non-diabetic patients?
Elevated perfusion pressure and pulsatile flow induce endothelial damage and reduce eNOS expression in saphenous veins from diabetic patients, which may have implications for bypass graft patency.
May warrant pressure-controlled perfusion during diabetic CABG vein harvest; leaves open effects on graft patency.
OBJECTIVE: This study was carried out to determine high pressure and pulsatile flow perfusion effects on human saphenous vein (HSV) segments obtained from diabetic and non-diabetic patients. METHODS: The veins were perfused with oxygenated Krebs solution for 3 h, with a pulsatile flow rate of 100 mL/min and pressures of 250 × 200 or 300 × 250 mmHg. After perfusion, veins were studied by light microscopy; nitric oxide synthase (NOS) isoforms, CD34 and nitrotyrosine immunohistochemistry and tissue nitrite/nitrate (NO(x)) and malondialdehyde (MDA) quantification. RESULTS: Light microscopy revealed endothelial denuding areas in all HSV segments subjected to 300 × 250 mmHg perfusion pressure, but the luminal area was similar. The percentage of luminal perimeter covered by endothelium decreased as perfusion pressures increased, and significant differences were observed between groups. The endothelial nitric oxide synthase (eNOS) isoform immunostaining decreased significantly in diabetic patients' veins independent of the perfusion pressure levels. The inducible NOS (iNOS), neuronal NOS (nNOS) and nitrotyrosine immunostaining were similar. Significant CD34 differences were observed between the diabetic 300 × 250 mmHg perfusion pressure group and the non-diabetic control group. Tissue nitrite/nitrate and MDA were not different among groups. CONCLUSIONS: Pulsatile flow and elevated pressures for 3 h caused morphological changes and decreased the eNOS expression in the diabetic patients' veins.
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Rosique et al. (2012) studied Diabetes. High pressure and pulsatile flow perfusion vs. Non-diabetic control group / lower pressure was evaluated on Morphological changes and eNOS expression. High pressure and pulsatile flow perfusion for 3 hours caused morphological changes and decreased eNOS expression in human saphenous veins from diabetic patients.
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