Estimates of Solutes Accumulating in Plants by 1H Nuclear Magnetic Resonance Spectroscopy

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A method is described for the estimation of betaines and proline in plant extracts using 1H nuclear magnetic resonance. After partial purification of the crude extract on a simple cation-exchange column the technique enables the simultaneous determination of glycinebetaine, choline and other N-methylammonium-containing compounds as well as proline. The method is simple and the results obtained compare favourably with those determined by other methods.