What does this research mean for the field?

Endothelial cells substantially magnify the antiplatelet effects of isosorbide dinitrate, completely arresting platelet aggregation under acute exposure compared to partial inhibition by the drug alone. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

April 1, 1987Circulation ResearchOpen Access

Antithrombogenic endothelial cell defense. Basal characteristics in cultured endothelial cells and modulation by short-term and long-term exposure to isosorbide nitrates.

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: Isosorbide dinitrate (ISDN) and metabolites vs. Control ECs / absence of drug. Primary outcome: Platelet aggregation inhibition.

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Key result

Acute isosorbide dinitrate achieves ~100% platelet aggregation inhibition in the presence of endothelial cells.

Why the study?

Does isosorbide dinitrate and its metabolites modulate antithrombogenic endothelial cell defense in cultured bovine endothelial cells?

Population

Bovine passage 0 cultured endothelial cells incubated with aggregating platelets and autologous plasma

Comparison

Isosorbide dinitrate, 2-isosorbide mononitrate… vs Control endothelial cells without drug

Design

Preclinical

Follow-up

5 days

Authors

FBF P Berenger JCJ. P. CanoUniversidad Nacional Santiago Antúnez de Mayolo PRP.H. RollandAix-Marseille Université

Discussion

Member takes

Implication

Isosorbide dinitrate may enhance endothelial antithrombotic effects in vitro; leaves open clinical translation for thrombosis prevention.

Structured PICO

Does isosorbide dinitrate and its metabolites modulate antithrombogenic endothelial cell defense in cultured bovine endothelial cells?

Population

In vitro experimental model using bovine cultured endothelial cells to evaluate platelet aggregation and the effects of isosorbide nitrates.

Intervention

Isosorbide dinitrate (ISDN), 2-isosorbide mononitrate (2-ISMN), and 5-isosorbide mononitrate (5-ISMN) (acute and 5-day exposure)

Comparator

Control endothelial cells without drug

Outcome

Platelet aggregation (antithrombogenic endothelial cell defense value)surrogate

Main Result

Absolute Event Rate: 100% vs 30%

Isosorbide dinitrate profoundly enhances the antithrombogenic properties of endothelial cells in vitro, an effect not seen with its mononitrate metabolites.

Cite This Study

Berenger et al. (1987) studied this question. Isosorbide dinitrate (ISDN) and metabolites vs. Control ECs / absence of drug was evaluated on Platelet aggregation inhibition. Under acute conditions, isosorbide dinitrate (5x10^-5 M) fully arrested platelet aggregation in the presence of endothelial cells, compared to 30% inhibition without endothelial cells.

synapsesocial.com/papers/6aa7792241a3eb417e4f250e https://doi.org/10.1161/01.res.60.4.612

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Antithrombogenic endothelial cell defense. Basal characteristics in cultured endothelial cells and modulation by short-term and long-term exposure to isosorbide nitrates.

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Antithrombogenic endothelial cell defense. Basal characteristics in cultured endothelial cells and modulation by short-term and long-term exposure to isosorbide nitrates.

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider