Key result
SHG imaging demonstrates progressive LV collagen deposition post-MI in a murine model.
Second harmonic generation imaging is a viable technique for characterizing collagen deposition and left ventricular remodeling in post-myocardial infarction murine models.
SHG collagen imaging is feasible in murine post-MI tissue; leaves open translation to clinical fibrosis assessment.
We utilized collagen specific second harmonic generation (SHG) signatures coupled with correlative immunofluorescence imaging techniques to characterize collagen structural isoforms (type I and type III) in a murine model of myocardial infarction (MI). Tissue samples were imaged over a four week period using SHG, transmitted light microscopy and immunofluorescence imaging using fluorescently-labeled collagen antibodies. The post-mortem cardiac tissue imaging using SHG demonstrated a progressive increase in collagen deposition in the left ventricle (LV) post-MI. We were able to monitor structural morphology and LV remodeling parameters in terms of extent of LV dilation, stiffness and fiber dimensions in the infarcted myocardium.
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Sahu et al. (2020) studied Myocardial infarction (murine model). Second harmonic generation (SHG) imaging was evaluated on Collagen deposition and structural morphology (LV dilation, stiffness, fiber dimensions). Second harmonic generation imaging demonstrated a progressive increase in collagen deposition in the left ventricle post-myocardial infarction in a murine model.
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