Validation method of organochlorine pesticides residues in water using gas chromatography–quadruple mass

In analytical chemistry laboratories, it is critical to have a sensitive method to provide applicable qualitative and quantitative data. This research aimed to determine validated optimized method and acceptable Profession Test (PT) results of 18 organochlorine pesticides (OCPs) using gas chromatography operating with quadruple mass spectrophotometer (GC–QMS) and liquid–liquid extraction (LLE) technique. This will be used for acquiring accreditation and checking alternative instruments such as gas chromatography operational with flame ionization detector (GC–FID). Ismailia canal were selected to investigate organochlorine pesticides content in freshwater used for irrigation and drinking purposes.The obtained data for limit of quantification (LOQ) varied from 0.159 to 0.254 with Relative Standard Deviation (RSD%) below 20% (4.18–6.12). method detection limit (MDL) ranged from 0.04 to 0.08 μg/ml and squared correlation coefficient (R2) ranged from 0.9992 to 0.9999. The study concluded that application to real samples has been successfully assessed by optimized method.