The theoretical and practical aspects of demonstrating autoantibodies to thyroid by immunofluorescence have been discussed elsewhere (Naim, 1962).The principle of the method is simple: the sera of patients with suspected autoimmune thyroiditis are applied to sections of human thyroid and any interaction of thyroid antigen with specific autoantibody is detected by means of a fluorescent anti-human-globulin conjugate.Unfixed fresh frozen sections of thyroid are employed for identifying the complement-fixing anti-cytoplasmic autoantibody, but these are inadequate for identifying anti-colloid activity as thyroid colloid is poorly retained on July 19,
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Nairn et al. (1962) studied this question.
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