Review highlights advances in small-molecule fluorescent probes for real-time biological imaging, indicating their growing clinical potential in surgery and precision medicine.
Key Points
To review recent engineering strategies for small-molecule fluorescent probes and assess their applications across molecular imaging, surgical guidance, and precision theranostics.
Surveyed chemical design strategies for small-molecule probes targeting specific biomolecules, biochemical pathways, and subcellular microenvironments.
Evaluated functional integration with complementary modalities, including real-time fluorescence and photoacoustic imaging platforms.
Analyzed clinical and preclinical applications in diagnostic screening, intraoperative surgical guidance, and therapeutic response monitoring.
Small-molecule fluorescent probes provide high sensitivity, structural versatility, and real-time visualization of cellular and subcellular microenvironments.
Probe platforms show effective diagnostic discrimination and clear utility for intraoperative fluorescence-guided tissue resection.
Integration with dual-modality techniques such as photoacoustic imaging enhances tissue penetration depth and spatial resolution for precision theranostics.