Developmental innovation of inferior ovaries orchestrated by KNOX1 in cucumber

Spatiotemporal omics was used to analyze the important time nodes and important traits (such as carpel, placenta, ovule, etc.) in the process of cucumber fruit development, and to study the gene expression regulatory network from carpel primordium formation to fruit seat fruit in the early stage of cucumber flower development, and to reveal the mechanism of fruit formation and development in vegetable crops. Starting from the key times of fruit development such as early placenta and carpel formation of cucumber, the molecular regulatory network and key genes of cucumber were studied through high-resolution spatial transcriptome, and the key genes that determine the formation of ovary were excavated. Then, combined with the high-precision data of other fruits of Cucurbitaceae, the reasons and regulatory mechanisms of fruit diversity of Cucurbitaceae were elaborated in detail, and the data were jointly analyzed to identify rare cell groups, draw high-precision cell types and maps in spatial background, explore the heterogeneity of cell groups, and solve the problems of low resolution and high noise of batch RNA sequencing, so as to analyze the gene expression system in the whole cucumber fruit development trajectory in a fine and detailed manner. It provides strong evidence for plant tissue development and morphogenesis.