In vitro study demonstrates that Enterococcus rotai extracellular vesicles suppress melanin synthesis and inflammation in skin cells, suggesting therapeutic potential for hyperpigmentation.
Key Points
To investigate the antioxidant, anti-inflammatory, and antimelanogenic effects of Enterococcus rotai-derived extracellular vesicles and elucidate their underlying molecular mechanisms.
Purified and characterized extracellular vesicles (CA6-ErEVs) from the Centella asiatica-associated lactic acid bacterium Enterococcus rotai using nanoparticle analyses.
Assessed free radical scavenging, anti-inflammatory markers in TNF-α-stimulated dermal fibroblasts, and pigment production in α-MSH-activated murine and human melanocytes.
CA6-ErEVs suppressed melanin synthesis and tyrosinase activity while downregulating MITF, tyrosinase, TRP-1, and TRP-2 in activated melanocytes.
Vesicles reduced intracellular reactive oxygen species, attenuated proinflammatory cytokine release, decreased matrix degradation, and enhanced procollagen synthesis in fibroblasts.
Intracellular cAMP levels decreased due to intrinsic phosphodiesterase activity in CA6-ErEVs, inhibiting the cAMP-MITF signaling cascade driving melanogenesis.