What question did this study set out to answer?

To assess the effects of individual and combined dietary supplementation of Withania somnifera and Asparagus racemosus on growth performance, digestive enzyme activity, and gene expression in Channa punctatus.

April 28, 2024Aquaculture and FisheriesOpen Access

Dietary supplementation of Withania somnifera and Asparagus racemosus enhances growth performance coupled with transcriptional modulation of growth and immune responsive genes in fish, Channa punctatus

View Full Paper

Ask AI

Bookmark

To assess the effects of individual and combined dietary supplementation of Withania somnifera and Asparagus racemosus on growth performance, digestive enzyme activity, and gene expression in Channa punctatus.
Acclimatized fish (14 ± 1 g; 11 ± 1 cm) were randomly distributed into 10 dietary groups (Control, S1-S3, A1-A3, and combined AS1-AS3) with n = 10 per group in triplicate over a 60-day feeding trial.
Growth metrics (FBW, WG%, FCR, HSI%, VSI%) and digestive enzyme activities (trypsin and chymotrypsin) were measured at 30 and 60 days.
Quantitative real-time PCR (qRT-PCR) was performed to analyze transcript levels of growth-related genes (gh, igf-1, igf-2a) and an immune-related gene (tgf-β) in liver and muscle tissues.
All experimental groups except S1 showed significant increases in weight gain percentage (P < 0.05) and significant decreases in feed conversion ratio (P < 0.05) compared to controls at 60 days.
Digestive enzyme activity peaked in group AS3, showing a 3.47-fold increase in trypsin and a 2.06-fold increase in chymotrypsin activity compared to controls after 60 days (P < 0.05).
Combined supplementation (AS3) significantly upregulated gh expression (4.86-fold in liver, 4.43-fold in muscle) along with elevated igf-1, igf-2a, and hepatic tgf-β transcripts (P < 0.05).

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Feeding trial demonstrates enhanced growth and immune gene expression in Channa punctatus, indicating aquaculture utility.

Cite This Study

Trivedi et al. (2024) studied this question.

synapsesocial.com/papers/6aa7c367bb118c90a59f1611 https://doi.org/10.1016/j.aaf.2024.03.007

View Full Paper

Ask AI

Bookmark

Dietary supplementation of Withania somnifera and Asparagus racemosus enhances growth performance coupled with transcriptional modulation of growth and immune responsive genes in fish, Channa punctatus

Key Points

Discussion

Implication

Cite This Study

Dietary supplementation of Withania somnifera and Asparagus racemosus enhances growth performance coupled with transcriptional modulation of growth and immune responsive genes in fish, Channa punctatus

Key Points

Discussion

Implication

Cite This Study

Keep reading

Keep reading