Key result
Rotavirus occurs in ~27% of children with acute gastroenteritis, predominantly as the G1P[8] genotype.
Why the study?
Rotavirus causes severe diarrhea worldwide and contributes to one-third of pediatric diarrheal cases in Thailand, motivating evaluation before impending national vaccination program implementation.
Case-Control (n=3,470)
Yes
Absolute Event Rate: 26.8% vs 1.6%
Rotavirus is highly prevalent in Thai children with acute gastroenteritis, with G1P[8] being the predominant genotype, providing baseline epidemiological data prior to national vaccination.
Establishes pre-vaccine baseline for rotavirus gastroenteritis in Thailand; leaves open post-introduction impact assessment.
Rotavirus has been one of the major etiological agents causing severe diarrhea in infants and young children worldwide. In Thailand, rotavirus contributes to one-third of reported pediatric diarrheal cases. We studied stool samples from 1,709 children with acute gastroenteritis and 1,761 children with no reported gastroenteritis whose age ranged from 3 months to 5 years from four different regions in Thailand between March 2008 and August 2010. The samples were tested for the presence of rotavirus by real-time reverse transcription–polymerase chain reaction (RT-PCR) amplification of vp6 gene and enzyme-linked immunosorbent assay. The positive samples were further characterized for their G and P genotypes (vp7 and vp4 genes) by conventional RT-PCR. From all four regions, 26.8% of cases and 1.6% of controls were positive for rotavirus, and G1P[8] was the most predominant genotype, followed by G2P[4], G3P[8], and G9P[8]. In addition, the uncommon genotypes including G1P[4], G1P[6], G2P[6], G2P[8], G4P[6], G9P[4], G9P[6], G12P[6], and G12P[8] were also detected at approximately 14% of all samples tested. Interestingly, G5P[19], a recombinant genotype between human and animal strains, and G1P7[5], a reassortant vaccine strain which is closely related to four human-bovine reassortant strains of RotaTeq™ vaccine, were detected in control samples. Data reported in this study will provide additional information on molecular epidemiology of rotavirus infection in Thailand before the impending national implementation of rotavirus vaccination program.
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Sakpaisal et al. (2019) conducted a case-control in Acute gastroenteritis (n=3,470). Acute gastroenteritis vs. No reported gastroenteritis was evaluated on Rotavirus positivity. Rotavirus was detected in 26.8% of children with acute gastroenteritis compared to 1.6% of controls without gastroenteritis, with G1P[8] as the predominant genotype.
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