Laboratory study demonstrates chromatography-free synthesis of 2′-O-methoxyethylguanosine at kilogram scale, indicating an efficient production route for oligonucleotide therapeutics.
An optimized process to synthesize DMT protected 2 ′-O-methoxyethylguanosine is described. A key step involves the enzymatic deamination of a mixture of alkylated products to selectively afford the desired material without resorting to chromatography for purification. This approach was scaled up to kilogram quantities for use in oligonucleotide therapeutics. Process Research on the Preparation of DMT Protected 2′-O-Methoxyethylguanosine for Oligonucleotide Synthesis in Therapeutic ApplicationsAll authorsShabbir Ali S. Taj, S. Narayanan, S. Suman Meenakshi, Yogesh S. Sanghvi, Bruce S. Ross & Vasulinga T. Ravikumarhttps://doi.org/10.1080/15257770802271748Published online:18 August 2008
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