Spectroscopic analysis resolves individual residues and minor amide cis conformations in chitin oligomers, highlighting new methods for profiling N-acetylglucosamine glycans.
N dimension and detailed chemical shifts of amide proton and nitrogen are reported for the first time. Additionally, all oligomers were analysed for the presence of the amide cis form and its corresponding chemical shifts were assigned. This study exploits the information that can be obtained from chitin oligomers with NMR spectroscopy and depicts methods for detailed analysis of glycans containing oligomers of N-acetylglucosamine.
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Wiesinger et al. (2024) studied this question.
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